Color Modification
Dilution (d1) Linkage test
Alternative Names: Isabella, Lilac, Blue, Mouse Grey, Deer Grey, Grey, Grey-Brown, Silver, Charcoal, Champagne, Cream

The Dilution variant “d1” causes a lightening or dilution of pigment, especially dark pigments (eumelanin). Dilute black pigment appears grey, and dilute chocolate pigment appears isabella (a creamy brown). Red or yellow pigment (phaeomelanin) may show mild lightening of color. The d1 variant is found in the MLPH gene, known as the D locus. It was previously reported as Dilution (Variant 1).
Breeds
Dilution (d1) Linkage test is common in:

Greater Swiss Mountain Dog

Polish Greyhound

Tibetan Mastiff (Do Khyi)

American Staffordshire Terrier

Cardigan Welsh Corgi

Newfoundland

Pembroke Welsh Corgi

Great Dane

Cane Corso

White Swiss Shepherd

Soft Coated Wheaten Terrier

Lacy Dog

Belgian Tervuren

Irish Terrier

Belgian Sheepdog

Neapolitan Mastiff

Weimaraner

Nova Scotia Duck Tolling Retriever

Rat Terrier

Italian Greyhound

Doberman Pinscher

Belgian Malinois

Whippet

German Shepherd Dog

American Hairless Terrier

Bouvier des Flandres

Redbone Coonhound

Chinook

Boerboel

French Bulldog

Pumi
More Info
Did you know?
Coat color dilution is caused by the clumping of pigment within the hair shaft. This results in sections of hair which are relatively transparent, giving the impression of a lighter color. Eye and nose color are also affected, with dilute dogs showing striking amber, blue-grey, or green-blue eyes. Although the Weimaraner is the best known for dilution, many breeds can display the trait.
How it works
To show coat color dilution, a dog must inherit two copies of a dilution variant, one from each parent. This can either be two copies of a particular variant, such as this one (d1) or two of any combination of dilution variants. This variant (d1) is the most common dilution variant in dogs. The test for d1 is a linkage test, that measures markers close to the d1 variant to determine the most likely d1 genotype. The test is 99.2% accurate based on a set of over 3000 breed and mixed breed dogs with a known d1 genotype.
Health implications
Some forms of dilution cause the dilute-colored hair shaft to be brittle, resulting in early breakage of the hair at or near the skin, which can cause skin irritation. White spotted portions of the coat are not affected. This condition is called color dilution alopecia. The genetic cause of this disorder currently remains unidentified.
Prevalence
5 in 10 dogs
have one or more copy of this genetic variant in our testing.
Technical Details
Gene | MLPH |
---|---|
Also Called | Dilution (D) Locus |
Variant | G>A |
Chromosome | 25 |
Coordinate | 48,121,642 |
All coordinates reference CanFam3.1
References & Credit
Credit to our scientific colleagues:
Drögemüller, C., Philipp, U., Haase, B., Günzel-Apel, A. R., & Leeb, T. (2007). A noncoding melanophilin gene (MLPH) SNP at the splice donor of exon 1 represents a candidate causal mutation for coat color dilution in dogs. Journal of Heredity, 98(5), 468–473. View the article